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Procedure of PCR products with exonuclease I and warmth-labile alkaline phosphatase enhances the visibility of blended bisulfite restriction Examination

(one) Utilizing sequences pasted in the webpage or uploaded for a FASTA file primers are created based on the person-adjustable parameters; PrimerDimer is embedded to predict possible dimerization between primers. (2) Picked primer pairs are validated applying bisulfite-PCR as well as the effectiveness of primer pairs are analysed making use of qPCR.

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